Table 2 Significant differences in intracellular cytokine, plasma cytokine, and high-performance liquid chromatography (HPLC) data between the moderate and severe group
From: Distinct cytokine patterns may regulate the severity of neonatal asphyxia—an observational study
Time | Moderate | Severe | |
|---|---|---|---|
Intracellular cytokines—cell prevalence data (% of parent population) | |||
CD4+ IL-1b+/CD4+ | 6 h | 3.52 (2.13–5.16) | 6.77 (3.18–10.26) |
CD4+ IL-1b+ CD49d+/CD4+ IL-1b+ | 6 h | 6.98 (4.61–9.32) | 4.08 (2.86–5.46) |
CD4+ TNF-a + CD49d+/CD4+ TNF-a+ | 6 h | 6.63 (4.47–13.45) | 3.52 (2.12–7.23) |
CD8+ IL-17+/CD8+ | 6 h | 5.26 (3.89–14.40) | 2.63 (1.75–5.18) |
CD4+ FoxP3+/CD4+ | 24 h | 2.35 (1.96–3.13) | 3.02 (2.60–4.13) |
CD4+ TNF-a+ CD49d+/CD4+ TNF-a+ | 72 h | 4.77 (3.43–7.70) | 9.75 (6.31–10.80) |
CD4+ IL-17+/CD4+ | 1 week | 3.08 (1.80–4.59) | 5.13 (3.40–13.76) |
Intracellular cytokines—mean fluorescence intensity (MFI) data (arbitrary unit) | |||
CD8+ IL-17+/CD8+ | 24 h | 1069 (639–3265) | 4187 (1274–6133) |
CD4+ IFN-g+/CD4+ | 72 h | 455 (150–770) | 887 (496–1427) |
CD4+ IL-17+/CD4+ | 72 h | 939 (566–1674) | 1760 (1614–3508) |
CD4+ TNF-a+/CD4+ | 1 month | 3281 (1752–4326) | 4729 (3959–6714) |
Plasma cytokines (pg/mL) | |||
G-CSF | 24 h | 19.85 (10.87–30.70) | 42.74 (22.27–131.3) |
IL-5 | 72 h | 1.37 (0.00–4.69) | 0.20 (0.00–0.46) |
IL-13 | 72 h | 2.35 (2.01–3.67) | 1.70 (1.40–2.56) |
IL-6 | 1 week | 21.06 (11.89–43.24) | 70.25 (33.73–134.1) |
G-CSF | 1 week | 13.33 (5.52–17.72) | 32.90 (16.65–94.76) |
HPLC results (uM) | |||
KYN | 1 month | 3.62 (2.72–4.47) | 2.28 (1.45–3.14) |