Fig. 12

PKD2 is mainly responsible for a motile PMM phenotype. PMM were cultured in PDL-coated 24-well plates and transduced by shPKD1 and shPKD2. Control vectors expressing scrambled shRNA were used as control. a Silencing efficacy was assessed 72 h post transduction by qPCR. b–d Cells were serum-starved overnight and treated with 0.1% BSA or 1 μM LPA for 24 h. Velocity, accumulated distance, and Euclidean distance were analyzed. e Serum-starved PMM incubated with 0.1% BSA or 1 μM LPA were lysed, RNA was isolated and reverse-transcribed, and the gene products indicated were analyzed by qPCR. Expression ratios are normalized to HPRT expression. Results of three separate experiments in triplicate are presented as mean + SD (*p < 0.05, ***p < 0.001, compared to DMSO; ^# p < 0.05, ^## p < 0.01, ^### p < 0.001, cells treated with the inhibitor plus LPA compared to LPA-treated cells; one-way ANOVA with the Bonferroni correction)