Fig. 14

CRT0066101 reduces the expression of LPA-induced M1 markers. BV-2 cells were incubated in the presence of DMSO, DMSO plus LPA (1 μM), or LPA (1 μM) plus CRT (1 μM) for 24 h. Cells were stained for iNOS, COX-2, Arg-1, or RELMα and visualized using confocal microscopy. Fluorescence intensity was quantitated with ImageJ (bar graphs in right panels). At least 50 cells out of three different areas per chamber were measured. Results (three independent experiments) are presented as mean + SD (*p < 0.05, **p < 0.01, ***p < 0.001; ^# p < 0.05, ^## p < 0.01, inhibitor compared to LPA-treated cells; one-way ANOVA with the Bonferroni correction). Scale bars = 20 μm