Fig. 3

Intracellular trafficking of PKD1 and PKD2 in response to LPA. a BV-2 cells were cultured on chamber slides, serum-starved overnight, and incubated in the presence of 0.1% BSA (control) or LPA (1 μM) for 24 h. Cells were fixed, permeabilized, blocked, and stained for tubulin, PKD2, and nuclei (Hoechst). b, c PMM were cultured on chamber slides and serum-starved overnight. Cells were incubated in the presence of 0.1% BSA (control) or LPA (1 μM) for 24 h and stained with tomato lectin, b PKD1 or c PKD2 antibodies, and nuclei (Hoechst). Images were obtained using a Leica confocal microscope. Scale bars = 20 μm