Fig. 7

LPA controls the expression of pro-migratory, pro-invasive, and pro-angiogenic genes via the LPA/LPAR5/PKD axis. PMM were cultured in 24-well plates, serum-starved overnight, and treated with DMSO, DMSO plus LPA (1 μM), and LPA (1 μM) plus TCLPA5 (5 μM) or CRT (1 μM). At the indicated time points, cells were scraped, RNA was isolated and reverse-transcribed, and the gene products indicated were analyzed by qPCR. Expression ratios were normalized to HPRT expression. Results of three separate experiments performed in triplicate are expressed as mean + SD (*p < 0.05, **p < 0.01, ***p < 0.001, compared to vehicle control; ^# p < 0.05, ^## p < 0.01, ^### p < 0.001, cells treated with each inhibitor plus LPA compared to LPA-treated cells; REST; pairwise re-allocation test)