Fig. 2From: EBI2 receptor regulates myelin development and inhibits LPC-induced demyelinationEBI2 deficiency results in transient delay in MBP expression. a Representative confocal images show mice cerebellar slices prepared from P10 mice and cultured for 1, 2, 4 to 5, 7 to 9, and 14 DIV. Myelin (MBP, green) staining is shown. Myelin development was determined by quantification of MBP staining density. Analysis showed significant differences in myelin development between one to two DIV. Images taken at ×10 magnification. Scale bar, 100 μM. b Graph showing quantification of MBP immunofluorescence in images such as those shown in (a). Data presented as mean ± SD (five to six animals per group), unpaired t test, **p < 0.01, ***p < 0.001 versus corresponding control. c Representative WBs show differential expression of MBP in whole brain lysates prepared from embryonic (E14 and E18) and postnatal (P1, P7, P14, and P30) WT and EBI2 KO mice. d, e Densitometric quantification of blots such as that shown in (c). Normalized WB data presented as mean ± SD (three to four WB data per time point; one animal per blot), unpaired t test, *** p < 0.001 versus corresponding controlBack to article page