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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: EBI2 receptor regulates myelin development and inhibits LPC-induced demyelination

Fig. 3

Downregulation or antagonism of EBI2 signaling staggers myelination. Representative confocal images show MBP expression (green) and NF light chain (red) in WT cerebellar slices prepared from P10 mice and cultured for a 9 DIV, b 14 DIV, and c 20 DIV with or without 7α25HC (1 μM) and NIBR189 (1 μM). Corresponding bar graphs shows quantification of MBP staining. There are no differences between non-treated and treated slices at 9 and 14 DIV. However, treatment of slices with 7α25HC or NIBR189 for 20 DIV inhibits normal myelin development in WT cerebellar slices as measured by MBP staining. Data presented as mean ± SD (n = 4 and 6), one-way ANOVA and Dunnett’s post-test, ** p > 0.01, *** p < 0.001 versus corresponding control. Images taken at ×10 magnification. Scale bar 100 μm (d). Treatment of human monocytes (U937) with 7α25HC (10 μM) for 1 h induces EBI2 (green) internalization. NIBR189 (10 μM) does not induce receptor internalization. Images were taken at ×40 magnification. Scale bar 20 μM

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