Fig. 4

7α25HC protects from LPC-induced demyelination. Representative confocal images show WT (a) and EBI2 KO (b) mice cerebellar slices treated with 7α25HC (1 μM) and/or NIBR189 (1 μM) ± LPC (0.4 mg/ml). Anti-MBP (green) and anti-neurofilament (NF, red) immunostaining is shown. Images were taken at ×10 magnification. Scale bar, 100 μM. c A corresponding bar graph shows quantification of myelin staining in WT slices such as those shown in (a). 7α25HC significantly protects from LPC-induced demyelination, and this protective effect is inhibited with the EBI2 antagonist NIBR189 indicating EBI2 mediated protection from demyelination. Data presented as mean ± SD (n = 4 and 8), one-way ANOVA and Tukey’s multiple comparison post-test, ** p < 0.01 versus corresponding control. d A bar graph shows that 7α25HC does not protect from LPC-induced demyelination in slices prepared from EBI2 KO animals confirming EBI2-dependent mechanism shown in (a and c). Data presented as mean ± SD (n = 4), one-way ANOVA and Tukey’s multiple comparison post-test, **p < 0.01 versus control. e Schematic diagram illustrating experimental design