Fig. 5
From: EBI2 receptor regulates myelin development and inhibits LPC-induced demyelination
7α25HC attenuates LPC-induced levels of IL1β and IL6 in cerebellar slices. a Schematic diagram illustrating experimental design. Treatment of slices with LPC (0.4 mg/ml) induces an increase in IL6 (b) and IL1β (c) protein release. 7α25HC (1 μM) attenuates LPC (0.4 mg/ml)-induced levels of IL6 (b) and IL1β (c). Co-treatment with EBI2 antagonist (NIBR189, 1 μM) blocks the 7α25HC-mediated effects on the levels of IL6 (b) and IL1β (c) indicating EBI2-dependent regulation of cytokine release. Treatment with NIBR189 alone did not alter IL6 (b) or IL1β (c) release. Data presented as mean ± SD (n = 3), one-way ANOVA and Bonferroni’s post-test, *** p < 0.001 versus corresponding control