Table 1 List of the antibodies used in the study
Antibody | Manufacturer | Ratio | Time (h) | Temperature (°C) |
|---|---|---|---|---|
Primary antibody | ||||
 Anti-arginase rabbit polyclonal | Abcam | 1:500 | 24 | 4 |
 Anti-CHIT-1 rabbit polyclonal | Abcam | 1:500 | 24 | 4 |
 Anti-COX-2 rabbit polyclonal | Abcam | 1:800 | 24 | 4 |
 Anti-GDNF mouse monoclonal | SCBT | 1:200 | 24 | 4 |
 Anti-Iba-1 goat polyclonal | Abcam | 1:800 | 24 | 4 |
 Anti-IFN-γ goat polyclonal | SCBT | 1:200 | 24 | 4 |
 Anti-IL-10 rabbit polyclonal | Abcam | 1:500 | 24 | 4 |
 Anti-IL-6 rabbit polyclonal | Abcam | 1:500 | 24 | 4 |
 Anti-iNOS rabbit polyclonal | Abcam | 1:800 | 24 | 4 |
 Anti-PGE2 rabbit polyclonal | Abcam | 1:500 | 24 | 4 |
 Anti-TNF-α mouse monoclonal | Abcam | 1:200 | 24 | 4 |
 Anti-VEGF rabbit polyclonal | Abcam | 1:500 | 24 | 4 |
Secondary antibody | ||||
 Anti-rabbit IgG (FITC-conjugated) | Chemicon | 1:200 | 2 | RT |
 Anti-rabbit IgG (FITC-conjugated) | Chemicon | 1:200 | 2 | RT |
 Anti-rabbit IgG (Cy3-conjugated) | Sigma-Aldrich | 1:200 | 2 | RT |
 Anti-mouse IgG (Cy3-conjugated) | Sigma-Aldrich | 1:200 | 2 | RT |
 Anti-goat IgG (Cy3-conjugated) | Sigma-Aldrich | 1:200 | 2 | RT |
 Anti-goat IgG (Cy3-conjugated) | Sigma-Aldrich | 1:200 | 2 | RT |
 Anti-rabbit IgG (Cy3-conjugated) | Sigma-Aldrich | 1:200 | 2 | RT |
 Anti-rabbit IgG (FITC-conjugated) | Chemicon | 1:200 | 2 | RT |
 Anti-rabbit IgG (Cy3-conjugated) | Chemicon | 1:200 | 2 | RT |
 Anti-rabbit IgG (FITC-conjugated) | Chemicon | 1:200 | 2 | RT |
 Anti-mouse IgG (Cy3-conjugated) | Sigma-Aldrich | 1:200 | 2 | RT |
 Anti-rabbit IgG (FITC-conjugated) | Chemicon | 1:200 | 2 | RT |