Fig. 2
Poldip2 deletion reduces blood-brain barrier disruption, increases the survival rate and motor function 24 h after tMCAO. a Comparison between the mean volume of the ischemic lesion in Poldip2+/+ and Poldip2+/− mice 24 h after tMCAO. The bar graph represents means ± SEM of 5-7 mice per group. b Mice received intraperitoneal injections of Evans blue 3 h before sacrifice. The brains were harvested and photographed before separation of hemispheres. Dye extravasation was quantified spectrophotometrically after overnight extraction and normalized to the corresponding hemisphere weight. The bar graph represents means ± SEM of three to six mice per group. Two-way ANOVA ***p < 0.001 vs. Poldip2+/+ sham and §§p < 0.01 vs. Poldip2+/+ ischemic mice. c Poldip2 depletion increases the survival rate 24 h after tMCAO. **p < 0.01. Bars represent % survival ± 10% confidence intervals, calculated using the modified Wald method. Postoperative deaths due to surgical complications were excluded from the data analyzed. d Comparison between the RotaRod performance in Poldip2+/+ and Poldip2+/− mice 6 and 24 h after cerebral ischemia induction. Data points represent the means ± SEM (time on RotaRod vs. pre-surgery in percent) of five to six mice per group. Two-way ANOVA ***p < 0.001 vs. Poldip2+/+ sham 6 h; &&&p < 0.001 vs. Poldip2+/+ sham 24 h; §p < 0.05 vs. Poldip2+/− ischemic mice 6 h and #p < 0.05 vs. Poldip2+/− ischemic mice 24 h