Fig. 6

Poldip2 mediates cytokine upregulation induced by tMCAO. Cytokine mRNAs and proteins were measured in the brain hemispheres, 24 h after sham surgery or tMCAO in Poldip2^+/+ and ^+/− mice. a TNF-α, IL-6, and TGF-β mRNAs were measured by quantitative RT-PCR. Bar graphs represent means ± SEM from six mice per group normalized to GAPDH. Two-way ANOVA **p < 0.01 vs. Poldip2^+/+ sham mice, §p < 0.05 vs. Poldip2^+/+ ischemic mice, and §§p < 0.01 vs. Poldip2^+/− sham mice. b TNF-α and IL-6 proteins were measured by multiplex high-sensitivity ELISA. Bar graphs represent means ± SEM from six mice per group. Two-way ANOVA **p < 0.01 vs. Poldip2^+/+ sham mice and §p < 0.05 vs. Poldip2^+/+ ischemic mice