Fig. 6

Knockdown of TG2 expression drives IL-4-activated THP-1 monocytes into a pro-inflammatory phenotype. Lentiviral particles were used to efficiently knockdown TG2 expression in THP-1 monocytes as confirmed both by a qPCR or b WB. To study the phenotype of TG2-KD cells, mRNA expression levels of c IL-1β, d TNF-α, and e IL-1ra were measured by qPCR in SCR or TG2-KD THP-1 monocytes activated for 24 h with IL-4 (50 ng/ml). Data presented are the mean values + SEM (standard error of the mean) of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. SCR scramble control, TG2-KD TG2 knockdown