Fig. 5

Tenuigenin inhibits NLRP3 inflammasome activation induced by MSU in BV2 microglia. a ELISA of IL-1β in supernatants from LPS-primed BV2 treated for 3 h with various doses of tenuigenin and then stimulated with MSU. b–f Immunoblot analysis of IL-1β in culture supernatants of LPS-primed BV2 treated for 3 h with various doses of tenuigenin and then stimulated with MSU (150 μg/ml) for 4 h, and immunoblot analysis of NLRP3, pro-IL-1β, pro-caspase-1, cleaved caspase-1 (p20), and IL-1β in lysates of those cells. All of the data were from the three independent experiments. Data are expressed as mean ± SEM, one-way ANOVA, **p < 0.01 in comparison with untreated control. ^# p < 0.05 and ^## p < 0.01 in comparison with LPS+MSU group