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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: In vivo evidence for the contribution of peripheral circulating inflammatory exosomes to neuroinflammation

Fig. 4

Exosomes isolated from LPS-treated mice increase CD68-positive cells. a Photomicrographs of the hippocampal (Hp), neocortical (Cx), and lateral ventricular (LV) areas from C57BL/6J mice. Mice were intravenously injected 1 mg of exosomes (in 200 μl PBS) isolated from the sera of donor mice 6 h after intraperitoneal injection of PBS or 10 mg/kg LPS. The recipient mice were euthanized after 24 h. Brain sections were stained with anti-CD68 antibody (green) and DAPI (blue). Scale bar, 50 μm. b Higher magnification images of the white-dotted box area from a. Figures are presented in the following sequence: upper left, CD68 (green); upper right, Iba1 (red); lower left, merge of CD68 and Iba1; and lower right, DAPI (blue). Co-localization of CD68 and Iba1 (white arrowheads). DG dentate gyrus. Scale bar, 20 μm. c Quantification of CD68-positive cells in the hippocampal, neocortical, and lateral ventricular areas. n = 3–5 per group. Data represent mean ± SEM; *P < 0.05

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