Fig. 8

Uptake of intravenously administered exosomes in the brain. a A schematic diagram of the experimental procedure. Exosomes isolated from the sera of the donor mice were labeled with green fluorescence by the Exo-Green kit and injected intravenously to the recipient mice. b Lower magnification images of the brain sections in different areas. DG dentate gyrus, D3V dorsal third ventricle, CA3 field CA3 of hippocampus. DAPI was shown in blue. Scale bar, 50 μm. c Co-localization of the Exo-Green-labeled exosomes and microglia. The recipient mice were euthanized 24 h after the injection of Exo-Green-labeled exosomes (1 mg in 200 μl PBS). Brain sections were stained with anti-Iba1 for microglia (red) and with DAPI for nuclei (blue). Scale bar, 20 μm. d Quantification of the exosome uptake ratio by microglia, neuron, and astrocyte. e Representative image of the liver section showing uptake of exosomes labeled with green fluorescence. Scale bar, 50 μm