Fig. 6

WWL70 treatment alleviated inflammatory response in CCI mouse spinal cord. The lumbar spinal cords 7 days post-CCI were dissected, RNA isolated, cDNA synthesized, and gene expression analyzed by qRT-PCR. Increased expression of IL-6 (a), IL-1β (b), and CCL2 (c) were observed in the CCI vehicle group and significantly decreased by WWL70 (10 mg/kg). (*p < 0.05, **p < 0.01, and ***p < 0.001 CCI/WWL70 compared to CCI/vehicle) (mean ± S.E.M., n = 10/group). WWL70 treatment after CCI also significantly increased IL-4 mRNA expression (d), **p < 0.01 (mean ± S.E.M., n = 10/group)