Fig. 6

Immunofluorescence staining of markers of the median eminence blood-brain barrier III. a Low magnification and b high magnification of immunofluorescence staining using primary antibodies against IGFBP2 (green) and GFAP (red). Specimens were obtained from bregma-anteroposterior − 2.06 to − 2.18. The images are representative of three independent experiments. 3v third ventricle, CTR control fed chow, GFAP glial fibrillary acidic protein, IGFBP2 insulin-like growth factor-binding protein 2, HFD high-fat diet, wk week. The main histological findings described in the text are highlighted by the yellow dashed ellipses