Fig. 7

Hypothalamic expression of proteins of the brain-derived neurotrophic factor system in mice fed a high-fat diet. a–c Real-time PCR determination of the expression of transcripts encoding for BDNF (a), NGFR (b), and TrkB (c) in the median eminence of mice. d, e Immunofluorescence staining using primary antibodies against IGFBP2 (green) and TrkB (red). Specimens were obtained from bregma-anteroposterior − 2.06 to − 2.18. The images are representative of three independent experiments. In a–c, n = 4; *p < 0.05 vs. respective control. 3v third ventricle, BDNF brain-derived neurotrophic factor, CTR control fed chow, IGFBP2 insulin-like growth factor-binding protein 2, HFD high-fat diet, NGFR nerve growth factor receptor, wk week. The main histological findings described in the text are highlighted by the yellow dashed boxes/ellipses