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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Dietary fats promote functional and structural changes in the median eminence blood/spinal fluid interface—the protective role for BDNF

Fig. 8

Immunoneutralization of BDNF. Schematic representation of the protocol employed to immunoneutralize BDNF (a). b Body mass during the experimental period. c Body mass variation during the experimental period. d Mean daily food intake during the experimental period. eh Real-time PCR determination of the expression of transcripts encoding for TNFα (e), IL6 (f), IGFBP2 (g), and GFAP (h) in the median eminence of mice. i Immunofluorescence staining using primary antibodies against IGFBP2 (green) and BDNF (red). Specimens were obtained from bregma-anteroposterior − 2.06 to − 2.18. The images are representative of three independent experiments. In eh, n = 4; *p < 0.05 vs. respective control. 3v third ventricle, aBDNF treated with immunoneutralizing antibody against BDNF, BDNF brain-derived neurotrophic factor, CTR control fed chow, GFAP glial fibrillary acidic protein, IGFBP2 insulin-like growth factor-binding protein 2, HFD high-fat diet, wk week. The main histological findings described in the text are highlighted by the yellow dashed boxes/ellipses

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