Fig. 3

The anti-excitotoxic effect of laquinimod does not involve the IL-1β-miR-142-3p-GLAST regulatory axis. a–d qPCR experiments performed on EAE cerebellar slices incubated with or without laquinimod (30 μM, 2 h) show that laquinimod did not modulate IL-1β mRNA (a), miR-142-3p (b), and GFAP mRNA (d) while significantly upregulated Slc1a3 mRNA coding for GLAST (c). e–g WB analysis for GLAST (e, f) and GFAP (e, g) protein levels indicate that both proteins were unchanged after laquinimod treatment on EAE cerebellar slices. All data are expressed as mean ± SEM and as fold change of EAE-vhl samples. Unpaired T test: **p < 0.01