Fig. 4

Cerebellar GLT-1 levels are unchanged during EAE. a, a’ Confocal images of cerebellar parasagittal slices stained for GLT-1 (red), GFAP (green), and nuclei (blue) show that the intensity of GLT-1 was not different between CFA and EAE slices; a diffuse labelling is visible in the molecular layer (ml), the Purkinjie cell layer (PCl) and, although less intensely, the granular layer (gl). Scale bar 50 μm (a) and 20 μm (a’). Nuclei were stained with DAPI in blue. b The graph shows Slc1a2 mRNA coding for GLT-1 analyzed by qPCR performed in EAE and CFA cerebellar slices. c, c’ Images of WB probed for GLT-1 and β-actin as internal control (c) and densitometric analysis of GLT-1 levels (c’) in EAE and CFA cerebellar slices. All data are expressed as mean ± SEM and as fold change of CFA samples