Fig. 5

Laquinimod acute incubation on cerebellar slices significantly increases GLT-1. a–b’ mRNA and protein analyses performed in EAE cerebellar slices incubated with or without laquinimod (30 μM, 2 h) indicate that laquinimod incubation did not change Slc1a2 mRNA (a) and significantly increased protein levels of GLT-1 (b, b’). c Immunostaining of cerebellar slices incubated with laquinimod or vhl shows that GLT-1 labelling (red) was increased in the molecular layer (ml) of EAE cerebellum in the presence of laquinimod compared to control slices (EAE-vhl), although the extent of astrogliosis was similar (green staining for GFAP). Scale bar 50 μm. High-magnification images in c’ show GLT-1 staining pattern in EAE-vhl and EAE-laquinimod with a global increase of GLT-1 labelling around PC soma and in the ml. Scale bar 20 μm. Nuclei were stained with DAPI in blue. d The graph shows mRNA expression of the neurotrophin BDNF performed by qPCR in both experimental groups. All data are expressed as mean ± SEM and as fold change of CFA samples. Unpaired T test, *p < 0.05