Fig. 10

Exposure to IgG-immune complex (IC) triggers endocytosis of FcγR in rat hippocampal cells. a, b DIV 7 hippocampal cells were incubated with rat anti-mouse IgG (magenta), followed by incubation with normal mouse IgG and transferrin (green) to label the entire endosomal pathway; nuclei are stained blue (DAPI). c, d Cells were pre-incubated with either FcγRIIb or FcγRIa antibodies to block FcγR before incubation with rat anti-mouse IgG, mouse IgG, and transferrin. a, c Representative photomicrographs of co-localization of rat anti-mouse IgG and transferrin (evident as white puncta). b, d Quantification of dual-labeled puncta by t test of log-transformed data (b) or one-way ANOVA followed by Tukey’s post hoc test (d). Data are presented as the mean ± SEM. Scale bar = 30 μm. Trsf = transferrin; IC = IgG immune complex