Fig. 2

Western blot analyses of FcγRIa and FcγRIIIa expression in cortical cell cultures. Representative blots showing FcγRIa (a) and FcγRIIIa (b) expression in cortical cells on DIV 2, 7, 14, and 21. Each sample was also probed for either GAPDH (FcγRIa immunoblots) or βIII-tubulin (FcγRIIIa immunoblots) as loading controls. MW molecular weight standards, N lysis buffer used as a negative control, P lysates from peritoneal macrophages used as a positive control. Densitometric analyses showed no significant effects of culture age on FcγRIa (c) or FcγRIIIa (d) expression. For each sample, the optical density of the FcγR immunopositive band was normalized to the optical density of the loading control. Data are presented as the fold-change (± SEM) in normalized optical density for each DIV relative to DIV 2 which is represented as the horizontal dotted line in the graphs