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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Fc gamma receptors are expressed in the developing rat brain and activate downstream signaling molecules upon cross-linking with immune complex

Fig. 8

IgG-immune complex (IC) concentration-dependently increases [Ca2+]i in primary hippocampal cells. DIV 7 hippocampal cell cultures were loaded with calcium indicator dye Fluo-4 prior to experimental manipulation. IgG-IC or vehicle were added 10 s after recordings started, and KCl (50 mM) was added at the end of each 2 min recording to verify cell viability. Changes in [Ca2+]i were calculated by measuring changes in fluorescence intensity (ΔF/F0). a Randomly selected traces of [Ca2+]i in KCl-responsive cells exposed to varying concentrations of IgG-IC (1:1 ratio of pre-complexed mouse IgG and rat anti-mouse IgG). b The percentage of cells that responded to IgG-IC or to the individual components of the immune complex (as evidenced by an increase in fluorescence > 50% of baseline fluorescence). The total number of cells that responded to IgG-IC or to the individual components of the immune complex (each at 100 μg/ml), divided by the total number of cells analyzed are provided in parentheses for each experimental condition. No significant differences were observed between groups. c Mean change in [Ca2+]i as determined by the average area under the curve (AUC) of the ΔF/F0 tracing for cells that responded to IgG-IC or to the individual components of the immune complex (each at 100 μg/ml). No significant differences were observed between groups. Whisker box plots depict 25th–75th percentile range of values (box edges), median (horizontal line in box), and 1st–99th percentile range of values (whiskers)

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