Fig. 9

IgG-immune complex (IgG-IC) increases Erk phosphorylation in primary hippocampal cell cultures. a Rat peritoneal macrophages were exposed for 5 min to varying concentrations of IgG-IC, lysed, and immunoblotted for phosphorylated Erk (pErk). The optical density of pErk immunoreactive bands, normalized to GAPDH, were 600-fold higher in peritoneal cells exposed to 10 μg/ml IgG-IC and > 10,000-fold higher in peritoneal cells exposed to 100 μg/ml IgG-IC compared to cells exposed to media alone. b DIV 7 hippocampal cells were exposed for 30 min to 10 or 100 μg/ml IgG-IC (1:1 ratio of pre-complexed mouse IgG and rat anti-mouse IgG), or to 10 or 100 μg/ml rat anti-mouse IgG. Cell lysates were separated by SDS PAGE and then immunoblotted for pErk, total Erk, and GAPDH. The optical density of bands immunoreactive for pErk and total Erk was normalized to the optical density of GAPDH immunoreactive bands in the same sample. The ratio of pErk to Erk is plotted as a percentage of vehicle controls. Data are presented as the mean ± SEM. MW molecular weight standards