Fig. 11

miR-124 attenuates MPTP-dependent apoptotic midbrain DA cell death in vivo. The mice were treated with stereotactic intraventricular treatment of miR-124 agomir for five consecutive days. Next, the mice received one intraperitoneal injection of MPTP-HCl per day for 5 days, whereas the control mice received saline injections. The agomir treatment was performed 2 days prior to the MPTP injection. Then, the mice were decapitated, and the midbrain was obtained 7 days after the last MPTP injection. Immunostaining (a) and stereological counts (b) of TH-positive neurons in the SNpc are shown. The scale bar represents 200 μm. Low magnification (scale bar, 200 nm) (c) and high magnification (scale bar, 100 nm) (d) are supported by confocal laser scanning microscopy of TH-positive neurons. Data are presented as the mean ± SD of three independent experiments. The fold change is statistically significant. **P < 0.01