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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: MicroRNA-124 regulates the expression of MEKK3 in the inflammatory pathogenesis of Parkinson’s disease

Fig. 2

Over-expression of miR-124 could effectively attenuate LPS-induced BV2 microglial activation. a Microglia were transfected with miR-124 mimics or miR-124 inhibitor. After 48 h, cells were harvested, and miR-124 expression was evaluated using RT-qPCR. bf BV2 cells were transfected with miR-124 mimics or miR-124 control for 48 h and then treated with LPS. After 24 h, cells were harvested, and the mRNA levels of the pro-inflammatory cytokines iNOS (b), IL-6 (c), TNF-α (d), TGF-β1 (e), and IL-10 (f) were evaluated using RT-qPCR. gk BV2 cells were transfected with miR-124 inhibitor or control inhibitor for 48 h. The mRNA levels of pro-inflammatory cytokines iNOS (g), IL-6 (h), TNF-α (i), TGF-β1 (j), and IL-10 (k) were determined using RT-qPCR following a 24-h incubation with LPS. The fold change was normalised by GAPDH RNA levels. Experiments performed in triplicate showed consistent results. Data are presented as the mean ± SD. The fold change is statistically significant. *P < 0.05, **P < 0.01

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