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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: MicroRNA-124 regulates the expression of MEKK3 in the inflammatory pathogenesis of Parkinson’s disease

Fig. 4

miR-124 targets MEKK3. a Alignment of the miR-124 binding site to Bim 3′-UTR is shown for different species as predicted using the TargetScan database. b, c BV2 cells were transfected with miR-124 mimics or ctrl mimics. After 48 h, cells were harvested, and the expression levels of MEKK3 mRNA (b) and protein (c) were evaluated using RT-qPCR and western blot analysis. The fold change was normalised by GAPDH levels. d Luciferase activity in HEK293 cells transfected with reporter constructs containing wild-type (WT) or mutated Bim 3′-UTR. The cells were cotransfected with indicated constructs and miR-124 mimics (100 nm) or control, and normalised levels of luciferase activity are shown. The results are presented as the mean ± SE from three independent experiments. The fold change is statistically significant. *P < 0.05, **P < 0.01. NS not significant

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