Fig. 2

Multi-modal detection of hippocampal neuro-inflammation in the rats exposed to CMS. a The representative PET images. b DPA-714 accumulation (n = 3/group). c Immunofluorescence staining of hippocampal sections from normal rats. TSPO (red), Iba-1 (green); scale bar, 50 μm. Arrows indicate the cells positive for Iba-1 and TSPO. d Iba-1/DAPI (red/blue) staining in the hippocampus; scale bar, 100 μm. e Qualification of Iba-1 immunofluorescence density (n = 4/group). f Western blot analysis of Iba-1 (n = 4/group). g Western blot analysis of CD11b (n = 4/group). All data are expressed as the mean ± SEM. *p < 0.05, **p < 0.01, compared to normal rats