Fig. 5

Changes of NOD-like receptor protein 3 (NLRP3) inflammasome in the hippocampus of CMS rats. a Immunofluorescence staining of hippocampal sections from normal rats. NLRP3 (red), Iba-1 (green); scale bar, 100 μm. Arrows indicate the cells positive for Iba-1 and NLRP3. b Western blot analysis of NLRP3. c PCR analysis of NLRP3. d Western blot analysis of ASC. e PCR analysis of ASC. f Western blot analysis of caspase-1 g PCR analysis of caspase-1 (n = 4–7/group). All data are expressed as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, compared to normal rats