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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: The role of HFE genotype in macrophage phenotype

Fig. 2

Iron handling protein concentrations in cellular lysates. Macrophages were plated in 100-mm2 tissue culture dishes (8 × 106 cells/dish). Following differentiation, the cells were incubated with control, 300 μM FAC, or 300 uM DFO supplemented media for 24 h. Subsequently, the media was replaced with control media for an additional 24 h and then harvested for analysis using an L-ferritin (a), H-ferritin (b), ferroportin (c), or transferrin-receptor (d) ELISA. The absorbance of the cell lysates was measured on a SpectraMax 340PC plate reader. Data represent the mean ± SEM from three independent experiments performed each time with triplicate samples and are compared to all treatment groups for statistical significance using one-way ANOVA. ***p < 0.0001

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