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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Age-related cognitive impairment is associated with long-term neuroinflammation and oxidative stress in a mouse model of episodic systemic inflammation

Fig. 5

Oxidative stress is associated with increased Nox2 expression in the aged brains after episodic inflammation. a Confocal images showing increased 4-hydroxynonenal (HNE) immunostaining in the hippocampus of aged mice after SI. Scale bar = 25 μm. b Western blots for HNE protein adducts show increased content of oxidized protein in the brains of aged mice after SI. Molecular weights shown in kilodaltons (KDa). c Quantification of HNE western blot bands intensity, normalized by actin bands. d Real-time PCR analysis of gene expression was carried out with whole brain mRNA from young and aged mice, naïve (control), and long term after episodic systemic inflammation (SI). Data were plotted as means ± SEM (N = 4–5 per group). Statistical analysis with Mann Whitney test (*P < 0.05 relative to young controls)

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