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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Strain-dependent effects of clinical echovirus 30 outbreak isolates at the blood-CSF barrier

Fig. 2

E-30 infection of HIBCPP cells leads to strain-dependent alteration of the TJ morphology involving ZO1. HIBCPP cells were infected with E-30 Bastianni, 13-311, 13-759, and 14-397 for 28 h and ZO1 staining was compared to uninfected controls. Cell layers were stained for nuclei with 4, 6-diamidino-2-phenylindole (DAPI) (shown here in blue), actin cytoskeleton with Phalloidin (shown here in yellow), the viral capsid protein 1 (VP1) (shown here in green) and ZO1 (red). Z-stack images were taken from regions with infected cells using an Apotome and represented through display of a cross-section through the z-plane of multiple slices in combination with a two-dimensional view from the top of the cell layer. For each strain we included a 400× enlarged image of an area which we considered a representative frame. The polarized cells are oriented with the apical side pointing up or to the right and basolateral downwards or to the left. Three images per strain showing different grouping of parallel staining in combination with a zoomed in view are displayed horizontally (column one: only ZO1; column two: DAPI, actin, and ZO1; column three: DAPI, VP1, and ZO1); E-30 strains are listed vertically. The images shown are representative examples of multiple stainings taken from six independent experiments each performed in duplicates

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