Fig. 3

E-30 infection of HIBCPP cells leads to strain-dependent alteration of TJ morphology involving occludin. HIBCPP cells were treated analogous to procedures described for Fig. 2. Cell layers were stained for nuclei with DAPI (shown here in blue), actin cytoskeleton with phalloidin (shown here in yellow), VP1 (shown here in green) and occludin (red). For detailed description of image acquisition and preparation please refer to Fig. 2. Three images per strain showing different grouping of parallel staining in combination with a zoomed in view are displayed horizontally (column one: only occludin; column two: DAPI, actin, and occludin; column three: DAPI, VP1, and occludin); E-30 strains are listed vertically. The images shown are representative examples of multiple stainings taken from six independent experiments each performed in duplicates