Fig. 1
From: Unconjugated bilirubin induces pyroptosis in cultured rat cortical astrocytes
Caspase-1 activation in astrocytes treated with UCB. a Caspase-1 activity was measured by cleaved caspase-1 (p20) using western blotting at different time points after astrocyte treatment with UCB. **p < 0.01, versus the control group (0 h) using a two-tailed Student’s t test with Dunnett’s test. b VX-765 treatment inhibited the activation of caspase-1 at 6 h after UCB stimulation. **p < 0.01, versus the control group; ##p < 0.01, versus the UCB group using one-way ANOVA with Bonferroni’s post hoc test. c Caspase-1 activity was significantly attenuated in the VX-765 group at 12 h after UCB treatment. **p < 0.01, versus the control group; ###p < 0.001, versus the UCB group using one-way ANOVA with Bonferroni’s post hoc test. The intensity of the bands was quantitated by scanning densitometry, standardized with respect to β-actin protein, and normalized to the values of the control group. Three independent experiments were performed in duplicate. Error bars, SD