Fig. 6
From: Unconjugated bilirubin induces pyroptosis in cultured rat cortical astrocytes
UCB induced the increased expression of NLRP3. Rat cortical astrocytes were treated as previously described for the indicated time periods. Total cell lysates were analyzed by western blotting. a Protein NLRP3 was measured by western blotting at different time points after astrocyte treatment with UCB. b The intensity of the bands was quantified by scanning densitometry, standardized with respect to β-actin protein, and normalized to the values of the control group (0 h). **p < 0.01, versus the control group (0 h) using a two-tailed Student’s t test with Dunnett’s test. Three independent experiments were performed in duplicate. Error bars, SD