Fig. 6

SDG decreases VLA-4 activation in monocytes. FACS analysis of monocytes stimulated by 12 nM LDV peptide (VCAM/ICAM consensus sequence) [28] or with 100 mM PMA for VLA-4 and LFA-1 activation, respectively, and treated with SDG (0, 10, and 50 μM) for 4 h. Representative histograms of VLA-4 activation (with conformational Abs HUTS-21) (a). Ten thousand events were collected. Data are presented as MFI. b Percent of conformational activation of integrin β1 (LFA-1) and integrin β2 (VLA-4) was calculated as described in “Methods” section. **p < 0.01