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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Histamine upregulates the expression of histamine receptors and increases the neuroprotective effect of astrocytes

Fig. 1

Identification of expression of histamine receptors in primary cultured astrocytes. a Immunolocalization of H1R, H2R, H3R, and H4R in astrocytes was performed by using antibodies against H1R, H2R, H3R, and H4R (green) and an antibody against the astrocyte marker GFAP (red). The results were imaged with a laser scanning confocal microscope. The blue staining represents DAPI. Scale bar = 25 μm. b Western blotting analysis of H1R, H2R, H3R, and H4R in the extracts of rat astrocytes. c The expression levels of the histamine H1, H2, H3, and H4 receptor subtypes were examined by quantitative RT-PCR. The data are presented as the mean ± s.e.m. of three independent experiments

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