Fig. 2

Trovafloxacin treatment attenuates blood brain barrier leakage in CCI-injured mice. a Representative images of perfused and fixed mice brains from sham and CCI-injured mice treated with vehicle or trovafloxacin (TVX) after 6 days post-CCI. b Representative western blot showing IgG protein levels from injury of sham and CCI-injured mice treated with vehicle or TVX 6 days post-injury. Bottom western blot corresponds to GAPDH. b Densitometric quantification of IgG levels for each group (n ≥ 5 per group) relative to their corresponding GAPDH expression levels. Note that sham mice treated with TVX are not displayed in the western blots. Values are represented as mean ± SEM. Two-way ANOVA followed by Tukey’s HSD test was used to determine statistical significance. *p < 0.01, sham vs. CCI group; **p < 0.01, CCI vehicle vs. CCI + TVX. No differences were found between sham vehicle and sham TVX (see also Additional file 1: Figure S1)