Fig. 8

Trovafloxacin does not affect downstream of ATP signaling. a Representative images of BV-2 cells that were seeded for transmigration assay and stimulated with C5a (10 nM) or ATP (200 μM) for 24 h in the presence or absence of trovafloxacin (TVX, 1 μM) or Brilliant Blue FCF (BBFCF, 5 μM). Cells were fixed and stained with crystal violet. b Quantification of transmigrated BV-2 cells at 24 h from five different fields at 20× per condition. Each experiment was repeated at least three times. One-way ANOVA with Tukey’s HSD test was used to measure statistical differences among groups. Values are expressed as mean ± SEM. *p < 0.05, C5a vs. C5a + trovafloxacin. **p < 0.05, C5a vs. C5a + BBFCF. No significant changes in the number of migrated cells were observed between ATP, ATP + TVX, and ATP + BBFCF (p > 0.05)