Fig. 2

Eosinophil percentage coordinates with Chi3l3 derived from inflammatory macrophages in brains of AC-infected mice. a Chi3l3⁺ cells were observed in a gate of SSC and Chi3l3⁺ (A), within the gate (A). CD45^hiF4/80⁺ and F4/80⁺CD11b⁺ populations are shown in (C) and (D), respectively. b Chi3l3⁺ cells were characterized by CCR2⁺ gate (C) or CX3CR1⁺ gate (F) separately. c (A–D) The co-localization of Chi3l3 or Iba1 (A), CD11b or Chi3l3 (B), and DAPI (C) in cerebrum of AC-infected mice. (D) is the merge of (A), (B), and (C). (E–H) Chi3l3 or Iba1 (E), CD11b or Chi3l3 (F), and DAPI (G) in the cerebellum of AC-infected mice. (H) is the merge of (E), (F), and (G). Scale bars indicate 20 μm. Scale bar in (I) indicates 5 μm. d The percentages of Chi3l3⁺CD45^loF4/80⁺cells in BMNCs of mice at 7, 14, and 21 dpi are presented. ^***P < 0.001, ^****P < 0.0001, AC-infected groups vs control group. Chi3l3⁺CD45^hiF4/80^pos cells in BMNCs of mice at 7, 14, and 21 dpi are presented. ^####P < 0.0001, AC-infected groups vs control group. ^$$P < 0.01, ^$$$P < 0.001, ^$$$$P < 0.0001. e The percentages of eosinophils in BMNCs of mice infected with AC at 7, 14, and 21 dpi. There were 5–10 mice per group. Ctrl indicates the concurrent control. *P < 0.05, ****P < 0.0001, AC-infected groups vs control group. ^###P < 0.001, AC-infected 14 dpi group vs AC-infected 7 dpi group. ^$$P < 0.01, AC-infected 21 dpi group vs AC-infected 14 dpi group. f The percentages of eosinophils in peripheral blood mononuclear cells (PBMCs) of mice infected with AC at 7, 14, and 21 dpi; 5–10 mice per group. Ctrl indicates the concurrent control. *P < 0.05, AC-infected groups vs control group. g Clophosome and saline were injected into BALB/c mice intravenously 1 day before of AC infection, followed by five intravenous challenges every 4 days. At 22 dpi, the brain samples were collected. h Brain Chi3l3 mRNA levels (g). i Histopathological changes of the brain (g) (scale bar, 50 μm). Data information: In (d-h), data are presented as mean ± SD. (Student’s t test)