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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Chi3l3: a potential key orchestrator of eosinophil recruitment in meningitis induced by Angiostrongylus cantonensis

Fig. 5

A positive feedback loop between Chi3l3 from macrophages and IL-13 from T lymphocytes in vitro. a The percent of IL-5+ cells in CD3+CD4+ spleen cells were detected by flow cytometry. b The percent of IL-13+ cells in CD3+CD4+ cells. *P < 0.05, AC-infected D21 (21 dpi) group vs control group. c, d Spleen cells isolated from normal mice and AC-infected mice were stimulated with sAg (25 μg/mL) or Chi3l3 (10 ng/mL) for 72 h in vitro. A summary of the percentages of IL-13+CD3+CD4+ cells following stimulation with sAg and Chi3l3 in normal mice. *P < 0.05, Chi3l3 group vs control group. e Western blot analysis of Chi3l3, JMJD3, CREB1, CEBPB, KLF4, Y641 phospho-STAT6, STAT6, and PPARγ of BMDMs in the presence of sAg, IL-13, sAg+IL-13, and LPS for 24 h. f Cells were cultured for 24 h in medium alone or treated with sAg, IL-13, or sAg+IL-13, and then, the OCR was monitored using the Seahorse Bioscience extracellular flux analyzer in real time. Dotted lines indicate incubation of cells with the indicated compounds. g Basal OCR of BMDMs cultured for 24 h in medium alone or treated with sAg, IL-13, or sAg+IL-13. **P < 0.01, ***P < 0.001, ****P < 0.0001, sAg group, IL-13 group, sAg+IL-13 group vs control group. #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001, sAg group and IL-13 group vs sAg+IL-13 group. h SRC of BMDMs cultured for 24 h in medium alone or treated with sAg, IL-13, or sAg+IL-13. **P < 0.01, ***P < 0.001, ****P < 0.0001, sAg group, IL-13 group, sAg+IL-13 group vs control group. #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001, sAg group and IL-13 group vs sAg+IL-13 group. i Maximal OCR of BMDMs cultured for 24 h in medium alone or treated with sAg, IL-13, or sAg+IL-13. **P < 0.01, ***P < 0.001, ****P < 0.0001, sAg group, IL-13 group, sAg+IL-13 group vs control group. #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001, sAg group and IL-13 group vs sAg+IL-13 group. Data information: In (ad, fh), data are presented as mean ± SD (Student’s t test)

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