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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Lymphotoxin β receptor-mediated NFκB signaling promotes glial lineage differentiation and inhibits neuronal lineage differentiation in mouse brain neural stem/progenitor cells

Fig. 1

Ltα1/β2 activates classical and non-classical NFκB signaling pathway in mouse neural stem/progenitor cells (NSCs/NPCs). a, b Adenovirus-mediated NFκB-firefly-luciferase reporter assay showing a comparison of classical and non-classical NFκB stimulators (a) and a dose response of Ltα1/β2 (b) in NSCs/NPCs. Dissociated NSCs/NPCs cultured from adult mouse brain subventricular zones (SVZ) were plated on 96-well plate and infected with adenovirus carrying NFκB firefly-luciferase at 50 multiplicity of infection for 24 h and treated with indicated cytokines for 24 h. Luciferase activity was measured using OneGlo luciferase kit. Data are expressed as relative fold changes compared with corresponding control. c, d Western blot analysis of nuclear extracts from SVZ NSCs/NPCs for the nuclear translocation of non-classical (RelB, p52) and classical (p65) NFκB pathways. NSCs/NPCs were treated with indicated cytokines for 4 h before preparation of nuclear extracts. TNF: tumor necrosis factor; LT: lymphotoxin; IL: interleukin. Lamin A/C served as nuclear protein loading control

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Journal of Neuroinflammation

ISSN: 1742-2094