Fig. 6

Transgenic inactivation of astroglial NFκB pathway inhibits constitutive and LT-induced astroglial lineage differentiation but favors neuronal lineage differentiation. a, b Quantitative analysis of neuronal lineage differentiation determined by Tuj1 (immature neurons) and DCX (neuroblasts). c, d Quantitative analysis of glial lineage differentiation determined by GFAP (astrocytes) or MBP (Oligodendrocytes). Multi-labeled fluorescent immunocytochemistry and confocal image analysis were performed at 1–6 days of differentiation from SVZ NSCs/NPCs of littermated wild-type (WT) and GFAP-dnIκBα transgenic mice (TG) in the presence or absence of LTα₁β₂ (100 ng/ml). The percentage of Tuj1⁺ or DCX⁺ (neurons/neuroblasts), GFAP⁺ (astrocytes) and MBP⁺ (oligodendrocytes) over DAPI-stained cells per field under × 40 objective was determined. Data from 8 fields per duplicate well of each experiment containing littermated WT and TG NSCs/NPCs in 16-well chamberslide from 3 paired animals were statistically analyzed by two-tailed student t-test. *p < 0.05 and **p < 0.01 indicate significant changes in LTα₁β₂ treatment as compared to corresponding control. ⁺p < 0.05 and ⁺⁺p < 0.01 indicate significant changes in the TG group as compared with corresponding WT group