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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Calcineurin A beta deficiency ameliorates HFD-induced hypothalamic astrocytosis in mice

Fig. 3

Ppp3cb deficiency decreases the number of Iba1 positive cells in the VMH of HFD-fed mice. Immunohistochemical staining for Iba1 in the ARC, DMH, and VMH of Ppp3cb WT (a) and KO (b) mice and quantitative analysis of Iba1-positive microglia (ce) in the ARC (c), VMH (d), and DMH (e). Means ± SEM; n = 6 WT and five KO mice (c), n = 6 mice per genotype (d, e); n = 4 pictures per biological replicate were quantified. Unpaired two-tailed Student’s t test. **p < 0.01

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