Fig. 5

IRE1α inhibition upregulated miR-17-5p expression post HI and. TXNIP is a target of miR-17-5p. a qPCR results showed the down-regulation of miR-17-5p in HI compared with naive group. (n = 4, *P < 0.05 compared with naive group). b q-PCR results showed IRE1α inhibition upregulated mir-17-5p expression at 6 h after HI. (n = 4, **P < 0.01 compared with sham group, *P < 0.05 compared with sham group, and ^#P < 0.05 compared with vehicle+HI group). c Sequence alignment showed putative miR-17-5p binding sites within the 3′-UTR of the TXNIP mRNA in rats. d, f The expression levels of TXNIP mRNA (d, n = 3) and protein (f, n = 4, *P < 0.05 compared with negative control or naive group) were reduced at 48 h after administration of miR-17-5p mimic. miR-17-5p mimic-0.05 or 0.5: Syn-rno-miR-17-5p miScript miRNA mimic (0.05 or 0.5 nmol/pup). e, g The expression levels of TXNIP mRNA (e, n = 3) and protein (g, n = 4, *P < 0.05 compared with negative control or naive group) were increased at 48 h after administration of miR-17-5p inhibitor. miR-17-5p inhibitor-0.1 or 1: Anti-rno-miR-17-5p miScript miRNA inhibitor (0.1 or 1 nmol/pup)