Fig. 3

SLDS promotes M2 microglial polarization following MCAO. a Representative double immunofluorescence staining for Iba1 (green) and CD16 (red) 14 days after cerebral ischemia in the cortex and striatum. Scale bar, 50 μm. b A higher magnification image of CD16/Iba1-positive cells. Scale bar, 25 μm. c Representative images of Iba1 (green) and CD206 (red) immunostaining 14 days after cerebral ischemia in the cortex and striatum. Scale bar, 50 μm. d A higher magnification image of CD206/Iba1-positive cells. Scale bar, 25 μm. Numbers of Iba1⁺ microglia (e), Iba1⁺/CD16⁺ M1 microglia (f), and Iba1⁺/CD206⁺ M2 microglia (g) were quantified. 4′6-diamidino-2-phenylindole (DAPI, blue). Data are expressed as mean ± SEM; n = 5 per group. *P < 0.05, ***P < 0.001 vs vehicle groups, by two-way ANOVA and Tukey’s test