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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Salidroside provides neuroprotection by modulating microglial polarization after cerebral ischemia

Fig. 6

The effect of SLDS-mediated microglial polarization mediated on neuronal survival following OGD. The M1 phenotype was induced using LPS (50 ng/ml) plus IFN-γ (20 ng/ml) and the M2 phenotype was induced by IL-4 (20 ng/ml) plus IL-13 (20 ng/ml). a Microglia cultured in transwell plates were treated with different induction factors and/or SLDS for 48 h and conditioned media was removed and added to OGD-treated or untreated neuronal cultures for 48 h (left). Neuronal survival was quantified using MAP2 expression (right); n = 12 per group. b Microglia were directly added OGD-treated or untreated neurons and cultured for 48 h (left). Neuronal survival was quantified using MAP2 expression (right). n = 12 per group. c Representative Iba-1 (green) and MAP2 (red) staining of OGD-conditioned neurons cocultured with M0, M1, or M2 microglia in a direct neuron-microglia culture system. Vehicle was added into neuronal cultures and neuron-microglia cocultures without SLDS treatment. Scale bar, 25 μm. DAPI (blue) was used as a nuclear marker. Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, by one-way ANOVA and Tukey’s test

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